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cd56 lymphocytes  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd56 lymphocytes
    Cd56 Lymphocytes, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd56+lymphocytes/PSA-NCAM+Antibody%2C+anti-human%2Fmouse%2Frat/pm39812178-40-18-26
    Average 96 stars, based on 42 article reviews
    cd56 lymphocytes - by Bioz Stars, 2026-09
    96/100 stars

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    FACS:

    Article Title: Human HKU1-reactive CD4 T cells are enriched for cytolytic potential that persists in older adults.
    Article Snippet: The emergence of severe acute respiratory syndrome coronavirus 2 increased interest in cellular immunity established by infections with human coronaviruses (HCoVs).. Using peripheral blood mononuclear cells from a cohort of human subjects collected prior to 2019, we assessed the abundance and phenotype of these CD4 T cells using cytokine enzyme-linked immunosorbent spot assays.. Unexpectedly, cytotoxic potential was uniquely enriched among HKU1-reactive CD4 T cells, as measured by quantification of granzyme-producing cells.

    Isolation:

    Article Title: Functional paralysis of human natural killer cells by alphaherpesviruses
    Article Snippet: PBMCs were isolated from healthy human donor buffy coats (provided by the Australian Red Cross Blood Service) by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and resuspended in complete RPMI medium (RPMI 1640 with L-glutamine [Lonza] supplemented with 10% human serum [Sigma-Aldrich]). .. CD56 + lymphocytes were isolated from PBMCs by MACS positive selection using CD56 MicroBeads, following manufacturer’s protocol (Miltenyi Biotech), and subsequently resuspended in complete RPMI medium. .. For isolation of NK cells by FACS sorting, CD56 + -selected lymphocytes were stained with fluorochrome-conjugated antibodies in FACS buffer, and sorted to >95% purity using a FACSAria IIu (BD Biosciences).

    Article Title: Varicella zoster virus productively infects human natural killer cells and manipulates phenotype
    Article Snippet: Healthy human donor buffy coats were obtained through the Australian Red Cross Blood Service from which PBMCs were isolated by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and resuspended in complete RPMI medium (RPMI 1640 with L-glutamine [Lonza] supplemented with 10% human serum [Sigma-Aldrich]) for subsequent experiments. .. For some experiments, where specified, CD56 + lymphocytes were isolated from PBMC by MACS positive selection using CD56 MicroBeads, according to manufacturer’s protocol (Miltenyi Biotech). ..

    Magnetic Cell Separation:

    Article Title: Functional paralysis of human natural killer cells by alphaherpesviruses
    Article Snippet: PBMCs were isolated from healthy human donor buffy coats (provided by the Australian Red Cross Blood Service) by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and resuspended in complete RPMI medium (RPMI 1640 with L-glutamine [Lonza] supplemented with 10% human serum [Sigma-Aldrich]). .. CD56 + lymphocytes were isolated from PBMCs by MACS positive selection using CD56 MicroBeads, following manufacturer’s protocol (Miltenyi Biotech), and subsequently resuspended in complete RPMI medium. .. For isolation of NK cells by FACS sorting, CD56 + -selected lymphocytes were stained with fluorochrome-conjugated antibodies in FACS buffer, and sorted to >95% purity using a FACSAria IIu (BD Biosciences).

    Article Title: Varicella zoster virus productively infects human natural killer cells and manipulates phenotype
    Article Snippet: Healthy human donor buffy coats were obtained through the Australian Red Cross Blood Service from which PBMCs were isolated by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and resuspended in complete RPMI medium (RPMI 1640 with L-glutamine [Lonza] supplemented with 10% human serum [Sigma-Aldrich]) for subsequent experiments. .. For some experiments, where specified, CD56 + lymphocytes were isolated from PBMC by MACS positive selection using CD56 MicroBeads, according to manufacturer’s protocol (Miltenyi Biotech). ..

    Selection:

    Article Title: Functional paralysis of human natural killer cells by alphaherpesviruses
    Article Snippet: PBMCs were isolated from healthy human donor buffy coats (provided by the Australian Red Cross Blood Service) by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and resuspended in complete RPMI medium (RPMI 1640 with L-glutamine [Lonza] supplemented with 10% human serum [Sigma-Aldrich]). .. CD56 + lymphocytes were isolated from PBMCs by MACS positive selection using CD56 MicroBeads, following manufacturer’s protocol (Miltenyi Biotech), and subsequently resuspended in complete RPMI medium. .. For isolation of NK cells by FACS sorting, CD56 + -selected lymphocytes were stained with fluorochrome-conjugated antibodies in FACS buffer, and sorted to >95% purity using a FACSAria IIu (BD Biosciences).

    Article Title: Varicella zoster virus productively infects human natural killer cells and manipulates phenotype
    Article Snippet: Healthy human donor buffy coats were obtained through the Australian Red Cross Blood Service from which PBMCs were isolated by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and resuspended in complete RPMI medium (RPMI 1640 with L-glutamine [Lonza] supplemented with 10% human serum [Sigma-Aldrich]) for subsequent experiments. .. For some experiments, where specified, CD56 + lymphocytes were isolated from PBMC by MACS positive selection using CD56 MicroBeads, according to manufacturer’s protocol (Miltenyi Biotech). ..

    Immunopeptidomics:

    Article Title: Herpes simplex virus antigens directly activate NK cells via TLR2, thus facilitating their presentation to CD4 T lymphocytes.
    Article Snippet: PBMCs were prepared by Ficoll-Hypaque (GE Healthcare Biosciences) density gradient. .. Depletion of CD8+ and CD56+ lymphocytes from PBMCs To examine the immunogenicity of Pam2Cys–gD2 peptides and whether Pam2Cys–gD2 peptides target only CD4 T lymphocytes as intended, CD8 T lymphocytes and CD56+ NK cells were positively depleted using CD8 and CD56 MicroBeads (Miltenyi Biotec), respectively. ..



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    Image Search Results


    Suppression of PBMCs proliferation by myeloid subsets sorted from BM or BM-MDSCs. CellTrace-labelled PBMCs activated with anti-CD3 and anti-CD28 were cultured in the presence of 1:1 ratio of the myeloid populations, unsorted (UNS), mature (CD11b + ) or immature (CD11b − , BM-MDSCs). Immunosuppression of these populations was evaluated on activated T cells, (gated as CellTrace + /CD3 + ) normalized on the control without myeloid cells. ( n = 20 independent experiments for pediatric patients, n = 20 for elderly patients) a Suppression was calculated by CellTrace profile assessed as the reduction of the proliferating CD3 + cells (those contained within generation two onward) in the co-culture condition as compared to T cells cultured alone, expressed as percentage. b Suppression was assessed by T cell number calculated by analyzing the absolute number of proliferating CD3 + cells by TruCount™ tubes. In both cases proliferation data were normalized assuming as 100% the proliferation rate of CD3 + cells cultured without myeloid cells

    Journal: Immunity & Ageing : I & A

    Article Title: Human MDSCs derived from the bone marrow maintain their functional ability but have a reduced frequency of induction in the elderly compared to pediatric donors

    doi: 10.1186/s12979-020-00199-5

    Figure Lengend Snippet: Suppression of PBMCs proliferation by myeloid subsets sorted from BM or BM-MDSCs. CellTrace-labelled PBMCs activated with anti-CD3 and anti-CD28 were cultured in the presence of 1:1 ratio of the myeloid populations, unsorted (UNS), mature (CD11b + ) or immature (CD11b − , BM-MDSCs). Immunosuppression of these populations was evaluated on activated T cells, (gated as CellTrace + /CD3 + ) normalized on the control without myeloid cells. ( n = 20 independent experiments for pediatric patients, n = 20 for elderly patients) a Suppression was calculated by CellTrace profile assessed as the reduction of the proliferating CD3 + cells (those contained within generation two onward) in the co-culture condition as compared to T cells cultured alone, expressed as percentage. b Suppression was assessed by T cell number calculated by analyzing the absolute number of proliferating CD3 + cells by TruCount™ tubes. In both cases proliferation data were normalized assuming as 100% the proliferation rate of CD3 + cells cultured without myeloid cells

    Article Snippet: Myeloid populations were isolated through magnetic sorting by the depletion of CD3 + /CD19 + /CD56 + lymphocytes, with a cocktail of immunomagnetic beads obtained by combining anti–human CD3, CD19, and CD56 beads (Miltenyi Biotec).

    Techniques: Cell Culture, Control, Co-Culture Assay