cd56 lymphocytes (Miltenyi Biotec)
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Cd56 Lymphocytes, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd56+lymphocytes/PSA-NCAM+Antibody%2C+anti-human%2Fmouse%2Frat/pm39812178-40-18-26
Average 96 stars, based on 42 article reviews
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FACS:Article Title: Human HKU1-reactive CD4 T cells are enriched for cytolytic potential that persists in older adults. Article Snippet: The emergence of severe acute respiratory syndrome coronavirus 2 increased interest in cellular immunity established by infections with human coronaviruses (HCoVs).. Using peripheral blood mononuclear cells from a cohort of human subjects collected prior to 2019, we assessed the abundance and phenotype of these CD4 T cells using cytokine enzyme-linked immunosorbent spot assays.. Unexpectedly, cytotoxic potential was uniquely enriched among HKU1-reactive CD4 T cells, as measured by quantification of granzyme-producing cells. Isolation:Article Title: Functional paralysis of human natural killer cells by alphaherpesviruses Article Snippet: PBMCs were isolated from healthy human donor buffy coats (provided by the Australian Red Cross Blood Service) by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and resuspended in complete RPMI medium (RPMI 1640 with L-glutamine [Lonza] supplemented with 10% human serum [Sigma-Aldrich]). .. Article Title: Varicella zoster virus productively infects human natural killer cells and manipulates phenotype Article Snippet: Healthy human donor buffy coats were obtained through the Australian Red Cross Blood Service from which PBMCs were isolated by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and resuspended in complete RPMI medium (RPMI 1640 with L-glutamine [Lonza] supplemented with 10% human serum [Sigma-Aldrich]) for subsequent experiments. .. For some experiments, where specified, Magnetic Cell Separation:Article Title: Functional paralysis of human natural killer cells by alphaherpesviruses Article Snippet: PBMCs were isolated from healthy human donor buffy coats (provided by the Australian Red Cross Blood Service) by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and resuspended in complete RPMI medium (RPMI 1640 with L-glutamine [Lonza] supplemented with 10% human serum [Sigma-Aldrich]). .. Article Title: Varicella zoster virus productively infects human natural killer cells and manipulates phenotype Article Snippet: Healthy human donor buffy coats were obtained through the Australian Red Cross Blood Service from which PBMCs were isolated by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and resuspended in complete RPMI medium (RPMI 1640 with L-glutamine [Lonza] supplemented with 10% human serum [Sigma-Aldrich]) for subsequent experiments. .. For some experiments, where specified, Selection:Article Title: Functional paralysis of human natural killer cells by alphaherpesviruses Article Snippet: PBMCs were isolated from healthy human donor buffy coats (provided by the Australian Red Cross Blood Service) by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and resuspended in complete RPMI medium (RPMI 1640 with L-glutamine [Lonza] supplemented with 10% human serum [Sigma-Aldrich]). .. Article Title: Varicella zoster virus productively infects human natural killer cells and manipulates phenotype Article Snippet: Healthy human donor buffy coats were obtained through the Australian Red Cross Blood Service from which PBMCs were isolated by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) and resuspended in complete RPMI medium (RPMI 1640 with L-glutamine [Lonza] supplemented with 10% human serum [Sigma-Aldrich]) for subsequent experiments. .. For some experiments, where specified, Immunopeptidomics:Article Title: Herpes simplex virus antigens directly activate NK cells via TLR2, thus facilitating their presentation to CD4 T lymphocytes. Article Snippet: PBMCs were prepared by Ficoll-Hypaque (GE Healthcare Biosciences) density gradient. .. Depletion of CD8+ and |
